Antibodies:

1D8/7A1

Sensitivity:

0.2ng/ml

Limit of Detection:

0.8ng/ml

Assay Performance:

Intra-assay CV% of 8.9; Inter-assay CV% of 30

Contents:

Monoclonal antibody 1D8, Universal   Allergen Standard, Biotinylated antibody 7A1; sufficient reagents for 10   plates.

Storage:

Store at 4°C.

Not Provided:

50mM Carbonate/Bicarbonate buffer, pH 9.6

  Phosphate Buffered Saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)

  Bovine Serum Albumin

  Streptavidin-Peroxidase

  1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:

Der p 2 allergen concentration  can   only be determined in  D. pteronyssinus samples using the components of   this kit. Not recommended for use with samples that potentially contain both   D. pteronyssinus and D. farinae species of dust mite. Use Der p 1 (product   code: EL-DP1) and Der f 1 (product code: EL-DF1) kits to quantify the   concentration of both house dust mite species in a sample.

Der f 2 allergen concentration is   underestimated using this kit. To quantify the concentration of Der f 2 in a   single species sample, the use of the Der f 2 kit (product code: EL-DF2) is   recommended.

Product Resources:

EL-D2 Certificate of Analysis

For research and commercial use in vitro:   not for human in vivo or therapeutic use.

Certificate of Analysis

Monoclonal Antibody:

1D8 (clone 1D8 C4 D8)

Vial:

Red Cap; 100μl

Immunogen:

Der p 2

Isotype:

Mouse IgG2A

Specificity:

Binds to a common epitope on mite Dermatophagoides spp.   Group 2 allergens, Der p 2 and Der f 2.

Purification:

From ascites by ammonium sulphate precipitation and   purified by affinity chromatography using Protein A. Single heavy and light   chain bands on SDS-PAGE.

Concentration:

1.35mg/ml in phosphate buffered saline, pH 7.4. Based   on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.

Certificate of Analysis

Monoclonal Antibody:

7A1 (clone 7A1 H1 G3)

Vial:

Brown Cap; 100μl

Immunogen:

Der f 2

Isotype:

Mouse IgG1

Specificity:

Binds to a common epitope on mite Dermatophagoides spp   Group 2 allergens (Der f 2 and Der p 2).

Purification:

Produced in ascites and purified by affinity   chromatography using Protein A. Single heavy and light chain bands on   SDS-PAGE.

Biotinylation:

Biotinylated and titrated for use in ELISA at 1/1000   dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered,   preservative free.

Certificate of Analysis

Allergen Standard:

Universal Allergen Standard

Vial:

White Cap; 400μl

Composition:

A formulation of eight purified natural allergens   prepared in 1% BSA/50% glycerol/PBS, pH 7.4.

Concentration/Calibration:

Universal Allergen Standard

Protein Measurement

Concentration (ng/ml)

Der p 1

Amino-acid analysis

2500

Der f 1

Amino-acid analysis

2500

Der p 2

Amino-acid analysis

1000

Fel d 1

Amino-acid analysis

1000

Can f 1

Amino-acid analysis

2500

Rat n 1

Amino-acid analysis

1000

Mus m 1

Amino-acid analysis

250

Bla g 2

Amino-acid analysis

2500

References:

1.  Heymann PW, Chapman MD, Aalberse   RC, Fox JW, Platts-Mills TAE.  Antigenic and structural analysis of   group II allergens (Der p II and Der f II) from house dust mites   (Dermatophagoides spp.).  J Allergy Clin Immunol 1989;83:1055-1067.

2.  Lombardero M, Heymann PW,   Platts-Mills TAE, Fox JW, Chapman MD.  Conformational stability of   B cell epitopes on Group I and Group II Dermatophagoides spp.   allergens.  J Immunol 1990;144:1353-1360.

3.  DeBlay F, Heymann PW, Chapman   MD, Platts-Mills TAE.  Airborne dust mite allergens:    comparison of Group II allergens with Group I mite allergen and cat allergen,   Fel d I.  J Allergy Clin Immunol 1991;88:919-926.

4.  Ovsyannikova IG, Vailes LD, Li   Y, Heymann PW, Chapman MD.  Monoclonal antibodies to Group II   Dermatophagoides spp. allergens:  Murine immune response, epitope   analysis and development of a two-site ELISA.  J Allergy Clin   Immunol 1994;94:537-546.

5.  Platts-Mills TAE, Vervloet D,   Thomas WR, Aalberse RC, Chapman MD.  Indoor Allergens and Asthma:   Report of the Third International Workshop.  J Allergy Clin   Immunol, 1997; 100:S1

(For complete reference list see EL-D2   Certificate of Analysis)